I have a read through the thread and comments, Jake, and have also seen some of your material previously.
To me, you write well in your preferred genre, so let's not have any more of this old: "haven't got the skills" malarkey.
There's a lot of good advice here and I know you know this, but this is a tough old game and it's often down to bloody-minded tenacity and adopting a not giving up spirit.
If you see a door in front of you bang on it again and again. Also, I don't think you should just query agents that appear to be you genre. I'm sure there are plenty more out there unaware that you are their next big client. They just don't know it yet and they won't either... unless you approach them too.
The phrase
what have you got to lose? springs to mind.
Publishing is a merry-go-round with agents and editors changing horses all the time. What's to say that an agent / publisher purporting to be (say) a crime fiction specialist, hasn't just appointed a new go-getting-keen-to-make-an-impression-agent / editor with a penchant for comedy fantasy?